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TestORDER CODE 113Clinical chemistry

Bilirubin, Direct.

FIRST, THE PRACTICAL DETAILS

At a glance.

Specimen
Serum
Container
Serum Separator Tube (SST) – 0.5 ml serum, free from hemolysis.
Required volume
Serum Separator Tube (SST) – 0.5 ml serum, free from hemolysis.
Routine turnaround
1-2 days

Before collection

None required

Timing, specimen options and instructions reflect the source snapshot.

FROM COLLECTION TO THE LAB

Specimen & handling

Specimen
Serum
Container
Serum Separator Tube (SST) – 0.5 ml serum, free from hemolysis.
Required volume
Serum Separator Tube (SST) – 0.5 ml serum, free from hemolysis.
Preparation
None required
Collection instructions
Serum Separator Tube (SST) – 0.5 ml serum, free from hemolysis.
Processing
Follow all precaution for handling biological material. Use all appropriate Personal Protective Equipment (PPE). Allow blood samples to clot (15 mins). Separate the serum from the cells by centrifuging for 10 minutes. Store serum at 2-8°C until analysis. Protect specimen from light as soon as possible. Exposure to direct sunlight can decrease bilirubin in samples by 50% with in one hour.
Storage
Refrigerated.
Stability
After separation of cells, When well protected from light, bilirubin in serum is stable for up to 3 days at 2 – 8°C ; Frozen: three months when stored at<-20°C in the dark.
Rejection criteria
Any sample that is mislabeled; Any broken or damaged tubes.
Technical information & codes
Methodology
Quantitative Spectrophotometry
CPT code
83021

Codes are shown for identification from the source snapshot. Confirm current coding and billing information with the laboratory.

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